Flow Cytometric Methods to Assess Allergic Disease

Multiparametric flow cytometry is a powerful technique that allows the quantification and characterization of heterogeneous populations of cells. Advances in fl ow cytometric instrumentation, software, and reagents are occurring at a rapid pace, and flow cytometric methods are increasingly being applied to better understand cellular responses associated with a diverse array of disease conditions. This chapter provides an overview of some common applications of flow cytometry in characterizing mouse models of allergic airway disease.